cDNA clone encoding a complete rabbit immunoglobulin kappa light chain of b4 allotype.
نویسندگان
چکیده
منابع مشابه
Isolation of a shark immunoglobulin light chain cDNA clone encoding a protein resembling mammalian kappa light chains: implications for the evolution of light chains.
The time of emergence of immunoglobulin kappa and lambda light (L) chains in evolution is unknown. An L chain cDNA clone was isolated from the nurse shark (Ginglymostoma cirratum), a cartilaginous fish, whose predicted variable (V) region amino acid sequence has up to 60% sequence identity to mammalian V kappa domains. Genomic analyses suggest a cluster-type gene organization for this L chain l...
متن کاملAmino-acid sequence of an allotype b4 light chain from a rabbit antibody to streptococcal carbohydrate.
The complete amino-acid sequence was determined for a rabbit-antibody light chain of allotype b4. The light chain was recovered from approximately 20 g of homogeneous antibody to group C streptococcal carbohydrate, which was obtained from an immunized rabbit. Several enzymic digestions were used to obtain the overlapping peptides necessary for the sequence determination. The L chain has 210 ami...
متن کاملNucleotide sequence of constant and 3' untranslated regions of a kappa immunoglobulin light chain mRNA of a homozygous b4 rabbit.
A homozygous a2/a2 and b4/b4 rabbit has been hyperimmunized with Micrococcus lysodeikticus. Poly(A)-containing RNA has been isolated from the spleen and translated in vitro, and translation products have been analyzed by NaDodSO4/polyacrylamide gel electrophoresis. Double-stranded cDNA has been synthesized from poly(A)-containing RNA template and inserted in the Pst I endonuclease site of plasm...
متن کاملDevelopment and characterization of polyclonal antibody against human kappa light chain in rabbit
Polyclonal antibodies against kappa light chain are used to diagnose diseases producing free light chain. The kappa and lambda light chains are products of immunoglobulin synthesis and released into the circulation in minor amounts such as serum, cerebrospinal fluid, urine and synovial fluid in normal condition. The purpose of this study was the production and purification of polyclonal immunog...
متن کاملRearrangement and expression of rabbit immunoglobulin kappa light chain gene in transgenic mice.
To determine whether a foreign unrearranged immunoglobulin gene can be functionally rearranged and expressed in vivo, a rabbit b9 kappa light chain gene construct containing a single germ-line kappa chain variable (V) region gene (V kappa), the five kappa chain joining (J) segments (J kappa), and the kappa chain constant (C) region germ-line gene (C kappa) was introduced into fertilized mouse e...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1983
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.80.14.4489